Volume 4, Issue 2, June 2020, Page: 62-68
Antioxidant Phlorotannin from Brown Algae Sargassum dupplicatum: Enzyme-assissted Extraction and Purification
Vu Ngoc Boi, Faculty of Food Technology, Nha Trang University, Nha Trang, Vietnam
Nguyen Thi My Trang, Faculty of Food Technology, Nha Trang University, Nha Trang, Vietnam
Dang Xuan Cuong, Organic Matterial from Marine Resource, Nhatrang Institute of Technology Application and Research, Vietnam Academy of Science and Technology, Nha Trang, Vietnam
Hoang Thai Ha, Faculty of Fisheries, Ho Chi Minh University of Food Industry, Ho Chi Minh, Vietnam
Received: Apr. 8, 2020;       Accepted: Apr. 26, 2020;       Published: May 19, 2020
DOI: 10.11648/j.wjfst.20200402.17      View  273      Downloads  129
The study focused on the enzyme-assisted extraction and the purification of antioxidant phlorotannin content from brown algae Sargassum dupplicatum commonly grown in Vietnam. Antioxidant activities were evaluated, consisting of total antioxidant activity and reducing power activity for the enzyme-assisted extraction. Antioxidant purified phlorotannin was analyzed total antioxidant activity, reducing power activity, DPPH free radical scavenging activity, and lipoxygenase inhibition activity. The treating condition of brown algae by enzyme consisting of enzyme kinds (termamyl enzyme, cellulose enzyme, viscozyme enzyme), the enzyme concentration (2.5, 5.0, 7.5, 10.0, 12.5, and 15.0% (v/v)), and the treating time of algae (1, 2, 3, 4, and 5 hours). Brown algae were then soaked in 96% ethanol with the ethanol-to-brown algae ratio of 30/1 (v/w) for 24 hours at the room temperature for antioxidant phlorotannin extraction that purified by using the liquid-to-liquid method and the gel chromatography of Sephadex LH 20. Antioxidant phlorotannin purification was determined to base on DEPT and 1H NMR spectrum. The results showed that 7.5% cellulase enzyme destroyed brown algae cell for 3 hours leading the best efficiency of antioxidant phlorotannin extraction from brown algae for 24 hours by 96% ethanol with the ethanol-to-brown algae ratio of 30/1 (v/w) at the room temperature. Phlorotannin content, total antioxidant activity, and reducing power activity corresponded to 4.45±0.11 mg phloroglucinol equivalent/g DW, 11.17±0.28 mg ascorbic acid equivalent/g DW, and 11.09±0.24 mg FeSO4 equivalent/g DW, respectively. Two fractions of antioxidant phlorotannin (17 to 28 and 36 to 45) were collected after purification, possessed 4.1 to 177.3 and 8.3 to 112.2 mg phloroglucinol equivalent per 1087.56 mg glue, respectively. Total antioxidant activity, reducing power, and DPPH free radical scavenging corresponded to 12.48 to 585.52 and 28.08 to 371.28 mg ascorbic acid equivalent/1087.56 mg glue, 15.6 to 765.44 and 33.28 to 486.72 mg FeSO4 equivalent/1087.56 mg glue, and 72.23 to 100% and 76.02 – 100%, respectively. Lipoxygenase enzyme inhibition activity got 62.83 to 87.4 µM and 66.19 to 87.09 µM linoleic acid equivalent/ 100µl extract, respectively. DEPT and 1H NMR spectrum showed phloroglucinol exist in two over fractions.
Antioxidant, Brown Algae, Enzyme, Extraction, Lipoxygenase, Phlorotannin, Purification
To cite this article
Vu Ngoc Boi, Nguyen Thi My Trang, Dang Xuan Cuong, Hoang Thai Ha, Antioxidant Phlorotannin from Brown Algae Sargassum dupplicatum: Enzyme-assissted Extraction and Purification, World Journal of Food Science and Technology. Special Issue: Marine Bio-Polymer: Bio-Activity, Extraction and Application. Vol. 4, No. 2, 2020, pp. 62-68. doi: 10.11648/j.wjfst.20200402.17
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Nuno, C. A., Marcelo, D. C., Artur, M. S. S. and Susana, M. C. (2019). Review-Brown macroalgae as valuable food ingredients. Antioxidants, 8: 365-91.
Dang, X. C., Vu, N. B., Tran, T. T. V. and Le, N. H. (2016). Effect of storage time on phlorotannin content and antioxidant activity of six Sargassum species from Nhatrang Bay, Vietnam. J Appl Phycol, 28 (1): 567-72.
Vu, N. B., Dang, X. C. and Phan, T. K. V. (2017). Effects of extraction conditions over the phlorotannin content and antioxidant activity of extract from brown algae Sargassum serratum (Nguyen Huu Dai 2004). Free Radicals and Antioxidants, 7 (1): 115-22.
Charlotte, J., Ann-Dorit, M. S., Susan, L. H., Casimir, C. A. and Ditte, B. H. (2019). Source, extraction, characterization, and applications of novel antioxidants from seaweed. Annu Rev Food Sci Technol, 10: 541-68.
Kanti, B. and Syed, I. (2009). Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev, 2 (5): 270-8.
Neeti, S. (2014). Free radicals, antioxidants and disease. Biol Med, 6: 214-9.
Lobo, V., Patil, A., Phatak, A. and Chandra, N. (2010). Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev, 4 (8): 118-26.
Vinay, K., Ahmed, A. K., Anu, T., Praveen, K. D. and UK, B. (2015). Role of oxidative stress in various diseases: Relevance of dietary antioxidants. Int J Phytoph, 4 (2): 126-32.
yong-xin, l., Isuru, W., Yong, l. and Kim, S.-J. (2011). Phlorotannins as bioactive agents from brown algae. Process biochem, 46 (12): 2219-24.
Imbs, T. I. and Zvyagintseva, T. N. (2018). Phlorotannins are polyphenolic metabolites of brown algae. Russ J Mar Biol, 44: 263-73.
Dang, X. C., Nguyen, X. H., Dinh, H. D., Le, T. M. T., Nguyen, V. T., Hoang, T. H., Dang, T. T. T. and Dang, X. C., Tannins: Extraction from plants. In: Alfredo A, editor. Tannins - Structural properties, biological properties and current knowledge: IntechOpen, 2019, pp. 1-20.
Maya, P. Enzyme-assisted extraction of phlorotannins from Sargassum and biological activities: Diponegoro University; 2017.
Michael, A. B., Alan, T. C., Stefan, K., Akira, P., Kjersti, S. and Masahiro, N. Nineteenth International Seaweed Symposium. Kobe, Japan: Springer Science & Business Media; 2009.
Mattio, L., Payri, C. and Verlaque, M. (2009). Taxonomic revision and geographic distribution of the subgenus Sargassum (Fucales, Phaeophyceae) in the western and central Pacific islands based on morphological and molecular analyses. J Phycol, 45 (5): 1213-27.
Dang, X. C., Bui, M. L., Tran, T. T. V., Vu, N. B., Le, N. H. and Dao, T. H. (2014). Preliminary purification for phlorotannin and antioxidant activity from extract of brown algae Sargassum serratum. Journal of Agriculture and Rural Development, 2: 71-6.
Prieto, P., Pineda, M. and Aguilar, M. (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem, 269 (2): 337-41.
Indu, H. and Seenivasan, R. (2013). In vitro antioxidant activity of selected seaweeds from southeast coast of India. Int J Pharm Pharm Sci, 5 (2): 474-84.
Bateman, D. F. and Basham, H. G., Degradation of plant cell walls and membranes by microbial enzymes. In: Heitefuss R and Williams PH, editors. Physiological Plant Pathology Encyclopedia of Plant Physiology. 4. Berlin, Heidelberg: Springer, 1976, pp. 316-55.
Zhili, M., Yi, H., Wen, H., Xi, F., Fang, Y. and Deyuan, L. (2019). Separation, identification, and antioxidant activity of polyphenols from lotus seed Epicarp. Molecules, 24: 4007-16.
Xuezhen, Z., Mengqi, Y., Lijian, D., Shan, H. and Xiaojun, Y. (2019). Isolation and purification of a neuroprotective phlorotannin from the marine algae Ecklonia maxima by size exclusion and high-speed counter-current chromatography. Mar Drugs, 17: 212-7.
A-Reum, K., Tai-Sun, S., Min-Sup, L., Ji-Young, P., Kyoung-Eun, P., Na-Young, Y., Jong-Soon, K., Jae-Sue, C., Byeong-Churl, J. and Dae-Seok, B. (2009). Isolation and identification of phlorotannins from Ecklonia stolonifera with antioxidant and anti-inflammatory properties. J Agr Food Chem, 57: 3483-9.
Erwan, A. G., Florian, L., Mélanie, H., Camille, J. and Valérie, S.-P., Chapter 7 - Extraction and purification of phlorotannins from brown algae. In: Dagmar BS and Solène C, editors. Natural products from marine algae: methods and protocols, methods in molecular biology. 1308. New York: Springer Science+Business Media, 2015.
Henry, M. M., Wilson, M. N., Martin, O. O., Ntevheleni, T. and Wilfred, T. M. (2017). Phlorotannins and a sterol isolated from a brown alga Ecklonia maxima, and their cytotoxic activity against selected cancer cell lines HeLa, H157 and MCF7. Interdiscip J Chem, 2 (2): 1-6.
Ning, L., Shabana, I. K., Shi, Q. and Xing-Cong, L. (2018). Synthesis and anti-inflammatory activities of phloroglucinol-based derivatives. Molecules, 23: 3232-9.
Browse journals by subject