Volume 3, Issue 1, March 2019, Page: 6-13
Study of Acute Toxicity and the Effect of the Aqueous Extract of a Formulation of Three Edibles Mushrooms on Oxidative Stress Induced in Rats
Etoundi Omgba Cunégonde Blanche, Department of Biochemistry, Faculty of Science, University of Douala, Douala, Cameroon
Kayo Tayou Cynthia Valère, Department of Biochemistry, Faculty of Science, University of Douala, Douala, Cameroon
Mbang Mbarga Audrey Judith, Department of Biochemistry, Faculty of Science, University of Douala, Douala, Cameroon
Piéme Constant Anatole, Department of Biochemistry and Physiological Sciences, Faculty of Medicine and Biochemical Sciences, University of Yaounde I, Yaounde, Cameroon
Received: Feb. 13, 2019;       Accepted: Mar. 20, 2019;       Published: May 15, 2019
DOI: 10.11648/j.wjfst.20190301.12      View  47      Downloads  18
Edible mushrooms, especially Pleurotus spp. contain a wide variety of biomolecules that can play a protective role against oxidative damage. We investigate the effect of formulation of the aqueous extract of three edibles mushrooms commonly consumed in Cameroon on oxidative stress induced in rats induced by lead acetate: Pleurotus pulmonarius, Pleurotus floridanus and Pleurotus sajor-caju. The formulation made of the three species (1w:1w:1w) were dissolved in distilled water for acute toxicity (at 2000 mg/kg of bw) and protective effect (at 400 mg/kg of bw for 21 days) against oxidative stress induced in vivo with lead acetate (35 mg/kg of bw for 3 days). Several parameters were investigated in the serum and organs homogenous such as lipid profile, hepatic and oxidative stress markers. No particular sign of toxicity of the extract were revealed. After lead administration, significant increases were found in serum transaminases activity, creatinine, and lipid profile associated with a decrease of serum protein content and High Density Lipoprotein. In addition, significant increase in lipid peroxidation expressed as the concentration of malondialdehyde (MDA) and decreased level of reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) activities in serum, liver and kidney showed oxidative stress due to lead exposure. The pre-treatment with aqueous extract of the mushroom’s formulation showed protective effects against lead induced hepato-renal damage by improving antioxidant parameters, as well as ascorbic acid used as a reference. In conclusion, these mushrooms may be used as a natural source of antioxidants for food supplements against oxidative stress.
Oxidative Stress, Acute Toxicity, Lead Acetate, Edible Mushrooms, Pleurotus spp
To cite this article
Etoundi Omgba Cunégonde Blanche, Kayo Tayou Cynthia Valère, Mbang Mbarga Audrey Judith, Piéme Constant Anatole, Study of Acute Toxicity and the Effect of the Aqueous Extract of a Formulation of Three Edibles Mushrooms on Oxidative Stress Induced in Rats, World Journal of Food Science and Technology. Vol. 3, No. 1, 2019, pp. 6-13. doi: 10.11648/j.wjfst.20190301.12
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Maja K, Anita K, Dragica J, Nina T, Jovana V, Predrag P, Miomir N, Miroslav M, Vrvic, Leo van G. Antioxidants of Edible Mushrooms. Molecules. 2015. 20, 19489-19525; doi: 10.3390/molecules201019489.
Kehili N, Saka S, Aouacheri O. The Safener effect of Nigel (Nigella sativa) against cadmium-induced toxicity in rats. Phototherapy. 2017. pp 1–10. DOI 10.1007/s10298-017-1099-y.
Sadaf K, Mir Z, Rupal S, Sravani A. Free Radicals: Implications in Etiology of Chronic Diseases and Their Amelioration through Nutraceuticals. Pharmacologia. 2015. 6: 11-20.
Arbaayah H, Umi K. Antioxidant properties in the oyster mushrooms (Pleurotus spp.) and split gill mushroom (Schizophyllum commune) ethanolic extracts, Mycosphere. 2013. 4 (4): 661–673.
Boonsong S, Wanwimol K, Pongtep W. Antioxidant activities of extracts from five edible mushrooms using different extractants. Agriculture and Natural Resources. 2016. 50 89e97.
Kozarski M, Klaus A, Niksic M, van Griensven L, Vrvic M, Jakovljevic D. Polysaccharides of higher fungi: Biological role, structure and antioxidative activity. Chem. Ind. 2014. 68, 305–320.
Loria-Kohen V, Lourenco-Nogueira T, Espinosa-Salinas I, Marin F, Soler-Rivas C, Ramirez de Molina A. Nutritional and functional properties of edible mushrooms: A food with promising health claims. J. Pharm. Nutr. Sci. 2014. 4, 187–198.
Djomene Y, Foudjet E, Fon D, Ninkwango T. The marketing of edible mushrooms in Cameroon. Scientific and Technical Review Forest and Environment of the Congo basin. 2017. Vol 8. pp. 65-71.
Ninkwango TA. The cultivation of oyster mushrooms. Cameroon (2nd edn.). 2007. p: 47.
OCDE. Test No. 425: Acute Oral Toxicity: Up-and-Down Procedure, OECD Guidelines for the Testing of Chemicals, Section 4, Éditions OCDE, 2008. Paris, https://doi.org/10.1787/9789264071049-en.
Bradford M. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Anal. Biochem. 1976. 72: 248-254.
Allain C et al. Blink. Chem 1974. 20, 470. Quoted by datasheet AGAPPE. Ref: 51403007.
Friedewald W et al. Clin. Chem. 1972. 18, 499. Quoted by datasheet AGAPPE. Ref: 51010001.
Mc Gowan M, Artiss J, Standbergh D, Zak B. Clin. Chem. 1983. 29, 538. Quoted by datasheet FUTURA SYSTEM GROUP. Ref: 2704.
Young D. Effects of diseases on clinical Lab. Tests, 4th ed AACC. 2001. Quoted by CYPRESS Diagnostics spec Sheet. Ref: HB0080.
Kaplan L, CFSP A. Clin Chem. Mosby Ed. 1996. Quoted by datasheet SGM ITALIA. Ref: 20360.
Misra H, Fridovich I. The role of superoxide anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem. 1972. 247 (10): 3170–3175.
Sinha A. Colorimetric Assay of catalase. Analytical Biochemistry. 1972. 47 (2): 389–94.
Ellman G. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959. 82: 70–77.
Benzie F, Iris F and Strain J. The Ferric Reducing Ability of Plasma (FRAP) as a Measure of ‘‘Antioxidant Power’’: The FRAP Assay. Analytical Biochemistry. 1996. 239, 70–76.
Wilbur K, Bernheim F, Shapiro O. The thiobarbituric acid reagent as a test for the oxidation of unsaturated fatty acid by various agents. Arch Biochem Biophysic. 1949. 24: 305–13.
Etoundi O, Mbang M, Tuem S, Gouado I. Study of Toxicity and Antidiabetic Activity of Ethanolic and Hydroethanolic Extracts of Pleurotus pulmonarius and the Aqueous Extract of Pleurotus floridanus. J Food Nutr Popul Health. 2017. Vol. 1 No 2: 20.
Anil K, Ramesh M, Monika B and Satendra K. Therapeutic Influence of Zinc and Ascorbic Acid against Lead induced Biochemical Alterations. Therapy. 2009. 64 (6): 383-388.
Azab E. Hepatoprotective effect of sesame oil against lead induced liver damage in albino mice: histological and biochemical studies. Am. J. Biosci. 2014. 2 1–11.
Samuel J, Herbert O, Mbagwu, Orish E. Lead Induced Hepato-renal Damage in Male Albino Rats and Effects of Activated Charcoal. Front Pharmacol. 2017. 8: 107.
Moussa S and Bashandy S. Biophysical and biochemical changes in the blood of rats exposed to lead toxicity. Rom. J. Biophys. 2008. 18 123–133.
Saad S, Ahlem B, Wassila A. The effect of lead acetate-induced oxidative stress on the glutathione enzyme system in rats. Ann Toxicol Anal. 2011. 23 (3): 139-145.
Azoz H and Raafat R. Effect of lead toxicity on cytogenisity, biochemical constituents and tissue residue with protective role of activated charcoal and casein in male rats. Aust. J. Basic Appl. Sci. 2012. 6 497–509.
Sahach V, Korkach I, Kotsiuruba A, Prysiazhna O. The inhibition of oxidative and nitrosative stresses by ecdysterone as the mechanisms of its cardio- and vasoprotective action in experimental diabetes type I. Fiziol Zh. 2008. 54: 46–54.
Montonen J, Boeing H, Fritsche A, Schleicher E, Joost H, Schulze M, et al. Consumption of red meat and whole-grain bread in relation to biomarkers of obesity, inflammation, glucose metabolism and oxidative stress. Eur J Nutr. 2013. 52 (1): 337–45.
Irene R. Health and nutritional properties of fungi. A report. Technological Centre for Research on Mushroom (CTICH). 2015.
Kozarski M, Klaus A, Vunduk J, Zizak Z, Niksic M, Jakovljevic D, Vrvic M, van Griensven L. Nutraceutical properties of the methanolic extract of edible mushrooms Cantharellus cibarius (French fries): primary mechanisms. Food Funct. 2015. 6, 1875–1886.
Gil-Ramirez A, Clavijo C, Palanisamy M, Soler-Rivas C, Ruiz-Rodriguez A, Marín F, Reglero G, Pérez M. Edible mushrooms as potential sources of new hypocholesterolemic compounds. Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products (ICMBMP7). 2011. 110.
Gil-Ramírez A, Clavijo C, Palanisamy M, Ruiz-Rodríguez A, Navarro-Rubio M, Pérez M, Marín F, Reglero G, Soler-Rivas C. Study on the 3-hydroxy-3-methyl-glutaryl CoA reductase inhibitory properties of Agaricus bisporus and extraction of bioactive fractions using pressurised solvent technologies. Journal of the Science of Food and Agriculture. 2013a. 93 (11): 2789-2796.
Gil-Ramírez A, Clavijo C, Palanisamy M, Ruiz-Rodríguez A, Navarro-Rubio M, Marín F, Reglero G, Soler-Rivas C. Screening of edible mushrooms and extraction by pressurized water (PWE) of 3-hydroxy-3-methyl-glutaryl CoA reductase inhibitors. Journal of Functional Food. 2013b. 5 (1): 244-250.
Fidge N. Fighting high cholesterol levels-lipid lowering drugs. The Medical Journal of Australia. 1993. 159: 815-819.
Bobek P and Galbavy S. Effect of pleuran (beta-glucan from Pleurotus ostreatus) on the antioxidant status of the organism and on dimethylhydrazine-induced precancerous lesions in rat colon. British Journal of Biomedical Science. 2001. 58: 164-168.
Jeong S, Yang B, Islam R, Koyyalamudi S, Pang G, Cho K, Song C. White, button mushroom (Agaricus bisporus) lowers blood glucose and cholesterol levels in diabetic and hypercholesterolemic rats. Nutrition Research. 2010. 30 (1): 49-56.
Shalan M, Mostafa M, Hassouna M, Sathishsekar D, Subramanian S. Antioxidant properties of Momordica Charantiab (bitter gourd) seeds on Streptozotocin induced diabetic rats. Asia Pac J Clin Nut. 2005. 14, 153–158.
Flora S, Flora G, Saxena G, Mishra M. Arsenic and lead induced free radical generation and their reversibility following chelation. Cell Mol. Biol. 2007. 53 26–47.
Ahamed M and Siddiqui M. Environmental lead toxicity and nutritional factors. Clin Nut. 2007. 26: 400–408.
Sadowska-Bartosz I, Galiniak S, Bartosz G. Polyphenols protect against glycoxidation proteins. Free Radic Biol Med. 2014. 75 1: S47.
Gupta M and Shari S. Lipid peroxidation and antioxidant status in patients with diabetic retinopathy. Ind. J. Physiol. Pharmacol. 2005. 49: 187-12.
Tariq S. Role of ascorbic acid in free radicals trapping and toxicity of lead from Biosystems. Mol Biotechnol. 2007. 37: 62-65.
Das K and Saha S. L-ascorbic acid and alpha tocopherol supplementation and antioxidant status in nickel- or lead-exposed rat brain tissue. J Basic Clin Physiol Pharmacol. 2010. 21: 325–346.
Browse journals by subject